Exploration
Accueil
Racine
Exploration
Accueil

Serveur d'exploration sur le peuplier

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.

Identifieur interne : 002C89 ( Main/Exploration ); précédent : 002C88; suivant : 002C90

The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.

Auteurs : G. Qi [République populaire de Chine] ; J. Lu ; P. Zhang ; J. Li ; F. Zhu ; J. Chen ; Y. Liu ; Z. Yu ; X. Zhao

Source :

RBID : pubmed:21332894

Descripteurs français

English descriptors

Abstract

AIMS

Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there is no report that B. thuringiensis is toxic to the adults of long-horned beetle, Batocera horsfieldi, a pest of poplar trees. This work aims to select a B. thuringiensis strain toxic towards the adults of Asian long-horned beetle.

METHODS AND RESULTS

A total of 504 B. thuringiensis strains were tested for the insecticidal activity to B. horsfieldi adults by artificial feeding. A strain of ZQ-89 was found with a high toxicity to B. horsfieldi adults. The rectified lethal rate of ZQ-89 to beetle was 55·33%. Additionally, the body weight and egg-hatching rate of beetle, respectively, decreased by 2·22 and 19·62% after being fed with ZQ-89. Further investigation found that the pure parasporal crystal had high toxicity to beetle adults. The ZQ-89 crystal protein was purified and analysed by peptide-mapping fingerprint and found it was highly homologous to Cry1Ac protein. The crystal protein gene was cloned and named cry1Ac89. The cry1Ac89 gene and its promoter were inserted into the plasmid pHT304 and then transformed into B. thuringiensis acrystalliferous strain BMB171. SDS-PAGE analysis showed the BMB171-Cry1Ac89 recombinant strain successfully expresses a 133-kDa recombinant crystal protein with highly lethal activity to B. horsfieldi adults.

CONCLUSIONS

The strain of ZQ-89 is highly toxic to the adults of long-horned beetle, and the crystal protein mainly contributes to the antipest role of this strain. The cry1Ac89 gene is a good candidate to be used for making transgenic trees or develop environment-friendly bioinsecticides against long-horned beetles such as B. horsfieldi in the future.

SIGNIFICANCE AND IMPACT OF THE STUDY

It is the first report of a B. thuringiensis strain toxic to the adults of Asian long-horned beetle, and the Cry1Ac protein is also firstly reported to be toxic to Coleopteran pests.


DOI: 10.1111/j.1365-2672.2011.04974.x
PubMed: 21332894


Affiliations:

Links toward previous steps (curation, corpus...)

Le document en format XML

<record>
<TEI>
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.</title>
<author>
<name sortKey="Qi, G" sort="Qi, G" uniqKey="Qi G" first="G" last="Qi">G. Qi</name>
<affiliation wicri:level="3">
<nlm:affiliation>Huazhong Agricultural University, Wuhan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Huazhong Agricultural University, Wuhan</wicri:regionArea>
<placeName>
<settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Lu, J" sort="Lu, J" uniqKey="Lu J" first="J" last="Lu">J. Lu</name>
</author>
<author>
<name sortKey="Zhang, P" sort="Zhang, P" uniqKey="Zhang P" first="P" last="Zhang">P. Zhang</name>
</author>
<author>
<name sortKey="Li, J" sort="Li, J" uniqKey="Li J" first="J" last="Li">J. Li</name>
</author>
<author>
<name sortKey="Zhu, F" sort="Zhu, F" uniqKey="Zhu F" first="F" last="Zhu">F. Zhu</name>
</author>
<author>
<name sortKey="Chen, J" sort="Chen, J" uniqKey="Chen J" first="J" last="Chen">J. Chen</name>
</author>
<author>
<name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name>
</author>
<author>
<name sortKey="Yu, Z" sort="Yu, Z" uniqKey="Yu Z" first="Z" last="Yu">Z. Yu</name>
</author>
<author>
<name sortKey="Zhao, X" sort="Zhao, X" uniqKey="Zhao X" first="X" last="Zhao">X. Zhao</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">PubMed</idno>
<date when="2011">2011</date>
<idno type="RBID">pubmed:21332894</idno>
<idno type="pmid">21332894</idno>
<idno type="doi">10.1111/j.1365-2672.2011.04974.x</idno>
<idno type="wicri:Area/Main/Corpus">002F23</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002F23</idno>
<idno type="wicri:Area/Main/Curation">002F23</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002F23</idno>
<idno type="wicri:Area/Main/Exploration">002F23</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title xml:lang="en">The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.</title>
<author>
<name sortKey="Qi, G" sort="Qi, G" uniqKey="Qi G" first="G" last="Qi">G. Qi</name>
<affiliation wicri:level="3">
<nlm:affiliation>Huazhong Agricultural University, Wuhan, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Huazhong Agricultural University, Wuhan</wicri:regionArea>
<placeName>
<settlement type="city">Wuhan</settlement>
<region type="région">Hubei</region>
</placeName>
</affiliation>
</author>
<author>
<name sortKey="Lu, J" sort="Lu, J" uniqKey="Lu J" first="J" last="Lu">J. Lu</name>
</author>
<author>
<name sortKey="Zhang, P" sort="Zhang, P" uniqKey="Zhang P" first="P" last="Zhang">P. Zhang</name>
</author>
<author>
<name sortKey="Li, J" sort="Li, J" uniqKey="Li J" first="J" last="Li">J. Li</name>
</author>
<author>
<name sortKey="Zhu, F" sort="Zhu, F" uniqKey="Zhu F" first="F" last="Zhu">F. Zhu</name>
</author>
<author>
<name sortKey="Chen, J" sort="Chen, J" uniqKey="Chen J" first="J" last="Chen">J. Chen</name>
</author>
<author>
<name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name>
</author>
<author>
<name sortKey="Yu, Z" sort="Yu, Z" uniqKey="Yu Z" first="Z" last="Yu">Z. Yu</name>
</author>
<author>
<name sortKey="Zhao, X" sort="Zhao, X" uniqKey="Zhao X" first="X" last="Zhao">X. Zhao</name>
</author>
</analytic>
<series>
<title level="j">Journal of applied microbiology</title>
<idno type="eISSN">1365-2672</idno>
<imprint>
<date when="2011" type="published">2011</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Amino Acid Sequence (MeSH)</term>
<term>Animals (MeSH)</term>
<term>Bacillus thuringiensis (genetics)</term>
<term>Bacillus thuringiensis (metabolism)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Bacterial Proteins (isolation & purification)</term>
<term>Bacterial Proteins (toxicity)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Coleoptera (drug effects)</term>
<term>Coleoptera (microbiology)</term>
<term>Endotoxins (genetics)</term>
<term>Endotoxins (isolation & purification)</term>
<term>Endotoxins (toxicity)</term>
<term>Female (MeSH)</term>
<term>Hemolysin Proteins (genetics)</term>
<term>Hemolysin Proteins (isolation & purification)</term>
<term>Hemolysin Proteins (toxicity)</term>
<term>Male (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Pest Control, Biological (MeSH)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (toxicity)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>Animaux (MeSH)</term>
<term>Bacillus thuringiensis (génétique)</term>
<term>Bacillus thuringiensis (métabolisme)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Coléoptères (effets des médicaments et des substances chimiques)</term>
<term>Coléoptères (microbiologie)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Endotoxines (génétique)</term>
<term>Endotoxines (isolement et purification)</term>
<term>Endotoxines (toxicité)</term>
<term>Femelle (MeSH)</term>
<term>Hémolysines (génétique)</term>
<term>Hémolysines (isolement et purification)</term>
<term>Hémolysines (toxicité)</term>
<term>Lutte biologique contre les nuisibles (MeSH)</term>
<term>Mâle (MeSH)</term>
<term>Protéines bactériennes (génétique)</term>
<term>Protéines bactériennes (isolement et purification)</term>
<term>Protéines bactériennes (toxicité)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (toxicité)</term>
<term>Séquence d'acides aminés (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>Bacterial Proteins</term>
<term>Endotoxins</term>
<term>Hemolysin Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en">
<term>Coleoptera</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr">
<term>Coléoptères</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Bacillus thuringiensis</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>Bacillus thuringiensis</term>
<term>Endotoxines</term>
<term>Hémolysines</term>
<term>Protéines bactériennes</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en">
<term>Bacterial Proteins</term>
<term>Endotoxins</term>
<term>Hemolysin Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr">
<term>Endotoxines</term>
<term>Hémolysines</term>
<term>Protéines bactériennes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
<term>Bacillus thuringiensis</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr">
<term>Coléoptères</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en">
<term>Coleoptera</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr">
<term>Bacillus thuringiensis</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en">
<term>Bacterial Proteins</term>
<term>Endotoxins</term>
<term>Hemolysin Proteins</term>
<term>Recombinant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr">
<term>Endotoxines</term>
<term>Hémolysines</term>
<term>Protéines bactériennes</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cloning, Molecular</term>
<term>Female</term>
<term>Male</term>
<term>Molecular Sequence Data</term>
<term>Pest Control, Biological</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Animaux</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Lutte biologique contre les nuisibles</term>
<term>Mâle</term>
<term>Séquence d'acides aminés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">
<p>
<b>AIMS</b>
</p>
<p>Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there is no report that B. thuringiensis is toxic to the adults of long-horned beetle, Batocera horsfieldi, a pest of poplar trees. This work aims to select a B. thuringiensis strain toxic towards the adults of Asian long-horned beetle.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>METHODS AND RESULTS</b>
</p>
<p>A total of 504 B. thuringiensis strains were tested for the insecticidal activity to B. horsfieldi adults by artificial feeding. A strain of ZQ-89 was found with a high toxicity to B. horsfieldi adults. The rectified lethal rate of ZQ-89 to beetle was 55·33%. Additionally, the body weight and egg-hatching rate of beetle, respectively, decreased by 2·22 and 19·62% after being fed with ZQ-89. Further investigation found that the pure parasporal crystal had high toxicity to beetle adults. The ZQ-89 crystal protein was purified and analysed by peptide-mapping fingerprint and found it was highly homologous to Cry1Ac protein. The crystal protein gene was cloned and named cry1Ac89. The cry1Ac89 gene and its promoter were inserted into the plasmid pHT304 and then transformed into B. thuringiensis acrystalliferous strain BMB171. SDS-PAGE analysis showed the BMB171-Cry1Ac89 recombinant strain successfully expresses a 133-kDa recombinant crystal protein with highly lethal activity to B. horsfieldi adults.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>CONCLUSIONS</b>
</p>
<p>The strain of ZQ-89 is highly toxic to the adults of long-horned beetle, and the crystal protein mainly contributes to the antipest role of this strain. The cry1Ac89 gene is a good candidate to be used for making transgenic trees or develop environment-friendly bioinsecticides against long-horned beetles such as B. horsfieldi in the future.</p>
</div>
<div type="abstract" xml:lang="en">
<p>
<b>SIGNIFICANCE AND IMPACT OF THE STUDY</b>
</p>
<p>It is the first report of a B. thuringiensis strain toxic to the adults of Asian long-horned beetle, and the Cry1Ac protein is also firstly reported to be toxic to Coleopteran pests.</p>
</div>
</front>
</TEI>
<pubmed>
<MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM">
<PMID Version="1">21332894</PMID>
<DateCompleted>
<Year>2011</Year>
<Month>08</Month>
<Day>01</Day>
</DateCompleted>
<DateRevised>
<Year>2018</Year>
<Month>12</Month>
<Day>01</Day>
</DateRevised>
<Article PubModel="Print-Electronic">
<Journal>
<ISSN IssnType="Electronic">1365-2672</ISSN>
<JournalIssue CitedMedium="Internet">
<Volume>110</Volume>
<Issue>5</Issue>
<PubDate>
<Year>2011</Year>
<Month>May</Month>
</PubDate>
</JournalIssue>
<Title>Journal of applied microbiology</Title>
<ISOAbbreviation>J Appl Microbiol</ISOAbbreviation>
</Journal>
<ArticleTitle>The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.</ArticleTitle>
<Pagination>
<MedlinePgn>1224-34</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1365-2672.2011.04974.x</ELocationID>
<Abstract>
<AbstractText Label="AIMS" NlmCategory="OBJECTIVE">Bacillus thuringiensis is toxic to many insects including Coleopteran pests. However, there is no report that B. thuringiensis is toxic to the adults of long-horned beetle, Batocera horsfieldi, a pest of poplar trees. This work aims to select a B. thuringiensis strain toxic towards the adults of Asian long-horned beetle.</AbstractText>
<AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">A total of 504 B. thuringiensis strains were tested for the insecticidal activity to B. horsfieldi adults by artificial feeding. A strain of ZQ-89 was found with a high toxicity to B. horsfieldi adults. The rectified lethal rate of ZQ-89 to beetle was 55·33%. Additionally, the body weight and egg-hatching rate of beetle, respectively, decreased by 2·22 and 19·62% after being fed with ZQ-89. Further investigation found that the pure parasporal crystal had high toxicity to beetle adults. The ZQ-89 crystal protein was purified and analysed by peptide-mapping fingerprint and found it was highly homologous to Cry1Ac protein. The crystal protein gene was cloned and named cry1Ac89. The cry1Ac89 gene and its promoter were inserted into the plasmid pHT304 and then transformed into B. thuringiensis acrystalliferous strain BMB171. SDS-PAGE analysis showed the BMB171-Cry1Ac89 recombinant strain successfully expresses a 133-kDa recombinant crystal protein with highly lethal activity to B. horsfieldi adults.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The strain of ZQ-89 is highly toxic to the adults of long-horned beetle, and the crystal protein mainly contributes to the antipest role of this strain. The cry1Ac89 gene is a good candidate to be used for making transgenic trees or develop environment-friendly bioinsecticides against long-horned beetles such as B. horsfieldi in the future.</AbstractText>
<AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">It is the first report of a B. thuringiensis strain toxic to the adults of Asian long-horned beetle, and the Cry1Ac protein is also firstly reported to be toxic to Coleopteran pests.</AbstractText>
<CopyrightInformation>© 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y">
<Author ValidYN="Y">
<LastName>Qi</LastName>
<ForeName>G</ForeName>
<Initials>G</Initials>
<AffiliationInfo>
<Affiliation>Huazhong Agricultural University, Wuhan, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y">
<LastName>Lu</LastName>
<ForeName>J</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Zhang</LastName>
<ForeName>P</ForeName>
<Initials>P</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Li</LastName>
<ForeName>J</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Zhu</LastName>
<ForeName>F</ForeName>
<Initials>F</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Chen</LastName>
<ForeName>J</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Liu</LastName>
<ForeName>Y</ForeName>
<Initials>Y</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Yu</LastName>
<ForeName>Z</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y">
<LastName>Zhao</LastName>
<ForeName>X</ForeName>
<Initials>X</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList>
<PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic">
<Year>2011</Year>
<Month>03</Month>
<Day>16</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo>
<Country>England</Country>
<MedlineTA>J Appl Microbiol</MedlineTA>
<NlmUniqueID>9706280</NlmUniqueID>
<ISSNLinking>1364-5072</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004731">Endotoxins</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D006460">Hemolysin Proteins</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance>
</Chemical>
<Chemical>
<RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C030956">insecticidal crystal protein, Bacillus Thuringiensis</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList>
<MeshHeading>
<DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D001413" MajorTopicYN="N">Bacillus thuringiensis</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D001517" MajorTopicYN="N">Coleoptera</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D004731" MajorTopicYN="N">Endotoxins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D006460" MajorTopicYN="N">Hemolysin Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D010572" MajorTopicYN="N">Pest Control, Biological</DescriptorName>
</MeshHeading>
<MeshHeading>
<DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData>
<History>
<PubMedPubDate PubStatus="entrez">
<Year>2011</Year>
<Month>2</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed">
<Year>2011</Year>
<Month>2</Month>
<Day>22</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline">
<Year>2011</Year>
<Month>8</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList>
<ArticleId IdType="pubmed">21332894</ArticleId>
<ArticleId IdType="doi">10.1111/j.1365-2672.2011.04974.x</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations>
<list>
<country>
<li>République populaire de Chine</li>
</country>
<region>
<li>Hubei</li>
</region>
<settlement>
<li>Wuhan</li>
</settlement>
</list>
<tree>
<noCountry>
<name sortKey="Chen, J" sort="Chen, J" uniqKey="Chen J" first="J" last="Chen">J. Chen</name>
<name sortKey="Li, J" sort="Li, J" uniqKey="Li J" first="J" last="Li">J. Li</name>
<name sortKey="Liu, Y" sort="Liu, Y" uniqKey="Liu Y" first="Y" last="Liu">Y. Liu</name>
<name sortKey="Lu, J" sort="Lu, J" uniqKey="Lu J" first="J" last="Lu">J. Lu</name>
<name sortKey="Yu, Z" sort="Yu, Z" uniqKey="Yu Z" first="Z" last="Yu">Z. Yu</name>
<name sortKey="Zhang, P" sort="Zhang, P" uniqKey="Zhang P" first="P" last="Zhang">P. Zhang</name>
<name sortKey="Zhao, X" sort="Zhao, X" uniqKey="Zhao X" first="X" last="Zhao">X. Zhao</name>
<name sortKey="Zhu, F" sort="Zhu, F" uniqKey="Zhu F" first="F" last="Zhu">F. Zhu</name>
</noCountry>
<country name="République populaire de Chine">
<region name="Hubei">
<name sortKey="Qi, G" sort="Qi, G" uniqKey="Qi G" first="G" last="Qi">G. Qi</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002C89 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002C89 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Bois
   |area=    PoplarV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:21332894
   |texte=   The cry1Ac gene of Bacillus thuringiensis ZQ-89 encodes a toxin against long-horned beetle adult.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:21332894" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a PoplarV1
Wicri

This area was generated with Dilib version V0.6.37.
Data generation: Wed Nov 18 12:07:19 2020. Site generation: Wed Nov 18 12:16:31 2020